Biosurfactant Production + Thesis

Biosurfactant Production + Thesis-41
Phospholipids are known to form major components of microbial membranes.

The different classes of biosurfactant find application in different industrial processes.

The attention given to the production of biosurfactants in recent times is mainly due to their potential utilization in food processing, pharmacology, cosmetics, oil exploration and exploitation industries, environmental management, and agriculture (Makkar and Cameotra 2002; Mulligan 2005).

Biosurfactant from Pseudomonas aeruginosa SB30 was used in the EXXON Valdex oil spill in Alaska with 1 % being enough to remove two times the oil on water at temperatures of 40 °C and 80 °C.

In 1990, a superbug (oil eating bug) was invented in the oil spill clean-up of the state of Texas in the USA.

The phylogenetic tree analysis of the 16S r RNA gene classified the isolate as K. The sequence obtained from the isolate has been deposited in Gen Bank under the accession number KT254060.1.

The result obtained from the study revealed high biosurfactant activity with a maximum E of 70 % by sodium dodecyl sulphate (SDS).The biosurfactant screening techniques employed were emulsification assay, emulsification index (E ), lipase activity, haemolytic assay, oil spreading, and tilted glass slide.The bacterial isolate was identified based on phenotypic, biochemical, and molecular means.One application of biosurfactant that is of interest to environmentalist is in environmental management and bioremediation.Biosurfactants have been successfully applied in the bioremediation of crude oil-polluted sites.Thin-layer chromatography (TLC) and gas chromatography mass spectrometry (GC–MS) analyses were used in the classification and characterization of the biosurfactant produced.The biosurfactant produced was applied on selected hydrocarbons to determine its emulsifying capacity.Considerable attention has been given to the use of biosurfactants in recent times because of their potential industrial and environmental applications and ecological friendliness.Hydrocarbon-polluted soils have been major sources of biosurfactant-producing bacteria; resultantly, this study had been aimed at isolating and characterizing biosurfactant produced by Klebsiella pneumoniae strain IVN51 isolated from hydrocarbon-polluted soil in Ogoniland, Nigeria.Various experiments with laboratory scale of sand-packed columns and field trials have successfully demonstrated the effectiveness of biosurfactants in microbial enhanced oil recovery (MEOR).The use of biosurfactants in MEOR can be implemented in two different ways as either an ex situ biosurfactant injection or in situ biosurfactant production to achieve an increase in oil recovery from subsurface reservoirs (Banat et al. Both of them require that the biosurfactants and their producing microorganisms are able to tolerate the harsh environmental conditions, such as high salinities, temperatures, and pressures.


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