Microfluidics Thesis

Microfluidics Thesis-23
The concept of a biofuel cell takes inspiration from the natural capability of biological systems to catalyse the conversion of organic matter with a subsequent release of electrical energy.Enzymatic biofuel cells are intended to mimic the processes occurring in nature in a more controlled and efficient manner.This presents a challenge for system builders who want to efficiently build and use purpose-made instruments for conducting scientific experiments.

The concept of a biofuel cell takes inspiration from the natural capability of biological systems to catalyse the conversion of organic matter with a subsequent release of electrical energy.

As energy consumption becomes a global issue, they emerge as important tools for energy generation.

The microfluidic platforms developed are intended to maximize the amount of electrical energy extracted from renewable fuels which are naturally abundant in the environment and in biological fluids.

In paper IV, a microfluidic chip was developed to simultaneously isolate these three mononuclear leukocyte cell types directly from whole blood.

The microfluidic method reduces the sample volume requirement and is a promising tool for latent HIV-1 reservoir study. Morshed, Kajsa Noyan, Aman Russom, Anders Sönnerborg, Ujjwal Neogi.

Furthermore, the optimized RNAscope technique performed better than RNAflow for transcription and translation competent latent HIV-1 reservoir identification.

The RNAscope was also found to be independent of the HIV-1 subtype and can be applied to patient samples at single cell level.

Instrumentino was eventually used in all of the projects in this thesis, and while it required a lot of invested time for its development, it saved a lot of time in running experiments afterwards.

The first CE systems built for this thesis were for a collaborative project about the use of a C4D cell array for following after separation processes, and comparing them to computer simulations.

This thesis focuses on instrumental advances in microfluidics-based capillary electrophoresis systems for achieving various goals.

Microfluidics systems and purpose-made scientific instruments in general may consist of many different hardware units, often made by different companies.

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